STAT6 Rabbit Polyclonal Antibody

CAT#: TA319167

Rabbit polyclonal STAT6 phospho Y641 antibody

Product Images

Western Blot of Rabbit anti-STAT6 pY641 antibody. Lane 1: unstimulated Hela cell lysate. Lane 2: IL-4 stimulated Hela cell lysate. Load: 10 ug per lane. Primary antibody: STAT6 pY641 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP goat anti-rabbit at 1:40,000 for 30 min at RT. Block: 5% Blotto, 30 min at RT. Predicted/Observed size: 94 kDa, 90 kDa. Other band (s): unspecific bands.

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  Immunohistochemistry of Rabbit anti STAT6 pY641 Antibody in human breast carcinoma pH 9 (left) with negative control (right) Tissue: Human breast carcinoma

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:32,000, WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: STAT6 phospho Y641 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y641 of human STAT6 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: signal transducer and activator of transcription 6
• Database Link:
  NP_001171549
  Entrez Gene 6778 Human
  UniProt ID P42226
• Synonyms: D12S1644; IL-4-STAT; STAT6B; STAT6C
• Note: Anti-STAT6 phospho Y641 Antibody is ideal to study STAT6. STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is involved in interleukin-4 signaling. It interacts with NCOA1 via its C-terminal LXXLL motif. Further it induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Following stimulation by IL-4 and IL-3, Stat6 is activated via phosphorylation at Tyr641 and translocates to the nucleus where it regulates cytokine-induced gene expression. STAT6 research areas include Immunology, Signal Transduction and Chromatin & Nuclear Signaling.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway