c Fos (FOS) Rabbit Polyclonal Antibody

CAT#: TA312211

Rabbit polyclonal FOS (Thr232) antibody(Phospho-specific)

Product Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody.The picture on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 5mins), using FOS (Phospho-Thr232) antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from COS7 cells (Lane 2), using FOS (Phospho-Thr232) Antibody. The lane on the left is treated with synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1: 20000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human FOS around the phosphorylation site of threonine 232 (V-A-TP-P-E).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Fos proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  NP_005243
  Entrez Gene 14281 MouseEntrez Gene 314322 RatEntrez Gene 2353 Human
  UniProt ID P01100
• Synonyms: AP-1; C-FOS; p55
• Note: FOS (Phospho-Thr232) antibody detects endogenous levels of FOS only when phosphorylated at threonine 232.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: B cell receptor signaling pathway, Colorectal cancer, MAPK signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway