Cyclin E1 (CCNE1) Rabbit Polyclonal Antibody

CAT#: TA311852

Rabbit polyclonal Cyclin E1 (Thr395) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from HeLa cells, treated with Paclitasel (1uM, 60mins), using Cyclin E1 (phospho-Thr395) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Cyclin E1 (phospho-Thr395) antibodyThe picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 395 (L-L-TP-P-P).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin E1
• Database Link:
  NP_001229
  Entrez Gene 898 Human
  UniProt ID P24864
• Synonyms: CCNE; pCCNE1
• Note: Cyclin E1 (phospho-Thr395) antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 395

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer