CD31 (PECAM1) Rabbit Polyclonal Antibody

CAT#: TA309798

Rabbit Polyclonal CD31/PECAM1 Antibody

Product Images

Flow Cytometry: CD31/PECAM-1 Antibody TA309798 - CD31/PECAM-1 Antibody was tested in WeHi cells with Dylight 488 (green) alongside a matched isotype control (black).

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  Simple Western: CD31/PECAM-1 Antibody TA309798 - Image shows a specific band for CD31/PECAM 1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody TA309798 - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: CD31/PECAM-1 Antibody TA309798 - Detection of CD31 in HUVEC cell lysates.

Specifications

Product Data

• Applications: FC, ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Frozen, Simple Western: 1:1000, Flow Cytometry: 1:1000, Immunohistochemistry: 1:50, Western Blot: 1:10000, Immunohistochemistry-Paraffin: 1:50, Immunocytochemistry/ Immunofluorescence: 1:10-1:500
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A genomic peptide made to an internal region of the human Pecam1 protein (within residues 100-300). [Swiss-Prot P16284]
• Formulation: PBS, 30% glycerol and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: platelet and endothelial cell adhesion molecule 1
• Database Link:
  NP_000433
  Entrez Gene 18613 MouseEntrez Gene 5175 Human
  UniProt ID P16284
• Synonyms: CD31; EndoCAM; endoCAM; GPIIA; PECA1; PECAM-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration