PHD3 (EGLN3) Rabbit Polyclonal Antibody

CAT#: TA309638

Rabbit Polyclonal HIF Prolyl Hydroxylase 3 Antibody

Product Images

Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody TA309638 - Analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong nuclear staining of PHD3 primarily in the cancer cells while the stromal cells were largely negative for this protein.

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  Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody TA309638 - EGLN3/PHD3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Specifications

Product Data

• Applications: ChIP, Electron Microscopy, ICC/IF, IHC, IP, WB
• Recommended Dilution: Chromatin Immunoprecipitation, Immunohistochemistry: 1:250-1:1000, Mass Spectrometry, Knockdown Validated, Electron Microscopy, Western Blot: 1:1000-1:2000, Immunocytochemistry/ Immunofluorescence: 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin: 1:250-1:1000
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to residues between 50-100 of human Prolyl Hydroxylase Domain-Containing Protein 3 using the numbering given in entry NP_071356.1 (GeneID 112399).
• Formulation: Tris-citrate/phosphate buffer, pH 7 to 8 and 0.1% Sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: egl-9 family hypoxia inducible factor 3
• Database Link:
  NP_071356
  Entrez Gene 54702 RatEntrez Gene 112399 Human
  UniProt ID Q9H6Z9
• Synonyms: HIFP4H3; HIFPH3; PHD3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma