PSMA7 Rabbit Polyclonal Antibody

CAT#: TA308944

Rabbit Polyclonal antibody to Proteasome 20S alpha 7 (proteasome (prosome, macropain) subunit, alpha type, 7)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308944 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. D: Hep G2. 12% SDS PAGE. TA308944 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using proteasome alpha 7 (TA308944) antibody at 1:100 dilution.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Chicken, Pig, Rat, Bovine, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 10 and 247 of Proteasome 20S alpha 7 (Uniprot ID#O14818)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kDa
• Gene Name: proteasome subunit alpha 7
• Database Link:
  NP_002783
  Entrez Gene 26444 MouseEntrez Gene 29674 RatEntrez Gene 5688 Human
  UniProt ID O14818
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9. [provided by RefSeq]
• Synonyms: C6; HEL-S-276; HSPC; RC6-1; XAPC7
• Note: Seq homology of immunogen across species: Chicken (96%), Pig (98%), Rat (98%), Bovine (99%), Xenopus tropicalis (97%)

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome