AMPK alpha 2 (PRKAA2) Rabbit Polyclonal Antibody

CAT#: TA308756

Rabbit Polyclonal antibody to AMPK alpha 2 (protein kinase, AMP-activated, alpha 2 catalytic subunit)

Product Images

Sample (30 ug of whole cell lysate). A:NIH-3T3. 7.5% SDS PAGE. TA308756 diluted at 1:1000.

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  AMPK alpha 2 antibody detects PRKAA2 protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract. B. 30 ug HeLa whole cell lysate/extract. C. 30 ug A375 whole cell lysate/extract. 7.5 % SDS-PAGE. AMPK alpha 2 antibody (TA308756) dilution: 1:

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  Immunohistochemical analysis of paraffin-embedded A549 xenograft, using AMPK alpha 2 (TA308756) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed A431, using AMPK alpha 2 (TA308756) antibody at 1:200 dilution.

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  AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Chicken, Pig, Rat, Bovine, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (Uniprot ID#P54646)
• Formulation: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62 kDa
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 2
• Database Link:
  NP_006243
  Entrez Gene 78975 RatEntrez Gene 108079 MouseEntrez Gene 5563 Human
  UniProt ID P54646
• Background: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]
• Synonyms: AMPK; AMPK2; AMPKa2; PRKAA
• Note: Seq homology of immunogen across species: Chicken (96%), Pig (99%), Rat (98%), Bovine (99%), Xenopus tropicalis (96%)

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy