Rad9 (RAD9A) Rabbit Polyclonal Antibody

CAT#: TA308749

Rabbit polyclonal antibody to Rad9 (RAD9 homolog A (S. pombe))

Product Images

Sample (30 ug of whole cell lysate). A: A431. B: Hela. 10% SDS PAGE. TA308749 diluted at 1:1000.

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  Rad9 antibody detects RAD9A protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. Rad9 antibody (TA308749) dilution: 1:1000

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  Rad9 antibody detects RAD9A protein by Western blot analysis. A. 30 ug C2C12 whole cell lysate/extract. 10 % SDS-PAGE. Rad9 antibody (TA308749) dilution: 1:500

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  Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Rad9 antibody (TA308749) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 (TA308749) antibody at 1:100 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 197 and 391 of Rad9
• Formulation: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: RAD9 checkpoint clamp component A
• Database Link:
  NP_004575
  Entrez Gene 5883 Human
  UniProt ID Q99638
• Background: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq]
• Synonyms: RAD9

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency