Epoxide hydrolase (EPHX1) Rabbit Polyclonal Antibody

CAT#: TA308516

Rabbit Polyclonal antibody to Epoxide hydrolase 1 (epoxide hydrolase 1, microsomal (xenobiotic))

Product Images

Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2. 10% SDS PAGE. TA308516 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1 (TA308516) antibody at 1:500 dilution.

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  EPHX1 antibody detects EPHX1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. EPHX1 antibody (TA308516) dilution: 1:500.

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  Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1 (TA308516) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Bovine, Pig, Rat)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 223 of EPHX1 (Uniprot ID#P07099)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53 kDa
• Gene Name: epoxide hydrolase 1
• Database Link:
  NP_001129490
  Entrez Gene 25315 RatEntrez Gene 2052 Human
  UniProt ID P07099
• Background: Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Synonyms: EPHX; EPOX; HYL1; MEH
• Note: Seq homology of immunogen across species: Pig (88%), Rat (86%), Bovine (88%)

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450