RRBP1 Rabbit Polyclonal Antibody

CAT#: TA307916

Rabbit Polyclonal antibody to Ribosome binding protein 1 (ribosome binding protein 1 homolog 180kDa (dog))

Product Images

Ribosome binding protein 1 antibody validation by siRNA knock-down. Upperpanel: Ribosome binding protein 1 antibody TA307916. Lower panel: Vinculin antibody. A. 30 ug HepG2 whole cell lysate/extract. B. 30 ug whole cell lysate/extract of RRBP1 siRNA#1-tra

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  Sample (30 ug of whole cell lysate). A:NIH-3T3. 5% SDS PAGE. TA307916 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1 (TA307916) antibody at 1:500 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1 (TA307916) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2500.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Ribosome binding protein 1 (TA307916) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:500.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Dog)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1183 and 1410 of Ribosome binding protein 1 (Uniprot ID#Q9P2E9)
• Formulation: 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 109 kDa
• Gene Name: ribosome binding protein 1
• Database Link:
  NP_001036041
  Entrez Gene 81910 MouseEntrez Gene 403809 DogEntrez Gene 6238 Human
  UniProt ID Q9P2E9
• Background: Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: 130; ES; ES130; hES; RRp
• Note: Seq homology of immunogen across species: Dog (83%)

Reference Data

• Protein Families: Druggable Genome, Transmembrane