p16INK4A (CDKN2A) Rabbit Polyclonal Antibody

CAT#: TA306428

Rabbit Polyclonal CDKN2A Antibody

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB: 1 - 4 µg/mL; IHC-P: 5 - 10 µg/mL; IF: 20 µg/mL.
  Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: CDKN2A antibody was raised against a 18 amino acid peptide from near the amino terminus of human CDKN2A.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: CDKN2A Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin-dependent kinase inhibitor 2A
• Database Link:
  NP_000068
  Entrez Gene 12578 MouseEntrez Gene 25163 RatEntrez Gene 1029 Human
  UniProt ID P42771
• Background: The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas. CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6. CDKN2A is expressed as at least three distinct isoforms.
• Synonyms: ARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer