Beclin 1 (BECN1) Rabbit Polyclonal Antibody

CAT#: TA306256

Rabbit Polyclonal Beclin-1 Antibody

Product Images

Western blot analysis of Beclin-1 in 293 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

• 

  Immunohistochemistry of beclin-1 in rat brain tissue with beclin-1 antibody at 2 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Beclin-1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human Beclin-1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: beclin 1
• Database Link:
  AAH10276
  Entrez Gene 8678 Human
  UniProt ID Q14457
• Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6).
• Synonyms: ATG6; beclin1; VPS30