IRF5 Goat Polyclonal Antibody

CAT#: TA302667

Goat Polyclonal Antibody against IRF5

Product Images

TA302667 (0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  HEK293 overexpressing human IRF5 and probed with TA302667 at 1ug/ml (mock transfection in lane B).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:32,000. WB: 0.1-0.3ug/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QGPWPMHPAGMQ, from the C Terminus of the protein sequence according to NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.9 kDa
• Gene Name: interferon regulatory factor 5
• Database Link:
  NP_116032
  Entrez Gene 27056 MouseEntrez Gene 296953 RatEntrez Gene 3663 Human
  UniProt ID Q13568
• Background: This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist. [provided by RefSeq]
• Synonyms: SLEB10
• Note: IHC: This antibody showed nuclear staining in Human Cervix, as confirmed by anonymous customer. ChIP: This antibody has been successfully used in human monocyte-derived dendritic cells.

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Toll-like receptor signaling pathway