SIRT4 Goat Polyclonal Antibody

CAT#: TA302610

Goat Polyclonal Antibody against SIRT4

Product Images

TA302610 (0.75ug/ml) staining of U937 (A), (2ug/ml) K562 (B) and (3ug/ml) U2OS (C), MCF7 (D), and U251 (E) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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  TA302610 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

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  TA302610 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

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  TA302610 (7µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

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  TA302610 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

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  TA302610 (7µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

• TA302610 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

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Specifications

Product Data

• Applications: IHC, PEP-ELISA, WB
• Recommended Dilution: ELISA: 1:32,000. WB 1-3µg/ml.
• Reactivities: Human, Rat (Expected from sequence similarity: Mouse, Dog, Pig, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence SRCGELLPLIDPC, from the C Terminus of the protein sequence according to NP_036372.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38812 Da
• Gene Name: sirtuin 4
• Database Link:
  NP_036372
  Entrez Gene 75387 MouseEntrez Gene 304539 RatEntrez Gene 477507 DogEntrez Gene 23409 Human
  UniProt ID Q9Y6E7
• Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. COMPLETENESS: complete on the 3' end.
• Synonyms: SIR2L4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors