APE1 (APEX1) Mouse Monoclonal Antibody [Clone ID: 13B8E5C2]

CAT#: TA301765

Mouse Monoclonal Antibody against APEX1 (13B8E5C2)

Product Images

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free TA301765 - APE Antibody (13B8E5C2) TA301765 - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDA MB 231.

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  Simple Western: APE Antibody (13B8E5C2) - BSA Free TA301765 - APE Antibody (13B8E5C2) TA301765 - Image shows a specific band for APE1 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

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  Immunohistochemistry-Paraffin: APE Antibody (13B8E5C2) - BSA Free TA301765 - APE Antibody (13B8E5C2) TA301765 - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.

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  Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free TA301765 - APE Antibody (13B8E5C2) TA301765 - HeLa cells stained TA301765 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

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  Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free TA301765 - APE Antibody (13B8E5C2) TA301765 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-APE (13B8E5C2) at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: APE Antibody (13B8E5C2) - BSA Free TA301765 - APE Antibody (13B8E5C2) TA301765 - Ovarian Cancer cell lines.

• Western Blot: APE Antibody (13B8E5C2) - BSA Free TA301765 - APE Antibody (13B8E5C2) TA301765 - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

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Specifications

Product Data

• Clone Name: 13B8E5C2
• Applications: ChIP, ELISA, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
• Recommended Dilution: Proximity Ligation Assay, Immunoblotting, ELISA, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Immunoprecipitation: 1:10 - 1:500, Immunohistochemistry-Frozen: 1:10-1:500, Immunohistochemistry: 1:100, Immunohistochemistry-Paraffin: 1:100, Western Blot: 1:100-1:2000, Simple Western: 1:25, Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Gel Super Shift Assays, Knockdown Validated
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: A fusion protein construct that contains the N-terminal region of human ACAT1.
• Formulation: PBS and 0.05% sodium azide
• Purification: Purified human APE/ref protein.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: apurinic/apyrimidinic endodeoxyribonuclease 1
• Database Link:
  NP_001632
  Entrez Gene 11792 MouseEntrez Gene 79116 RatEntrez Gene 328 Human
  UniProt ID P27695
• Background: The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.
• Synonyms: APE; APE1; APEN; APEX; APX; HAP1; REF1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair