RAD50 Rabbit Polyclonal Antibody [Clone ID: zinc hook domain]

CAT#: TA301492

Rabbit Polyclonal Antibody against RAD50 (zinc hook domain)

Product Images

Simple Western: Rad50 Antibody TA301492 - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Western Blot: Rad50 Antibody TA301492 - Detection of Rad50 using TA301492. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.

Specifications

Product Data

• Clone Name: zinc hook domain
• Applications: Simple Western, WB
• Recommended Dilution: Immunoprecipitation: 1-5ul, Western Blot: 1:1000-1:10000, Simple Western: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human Rad50 zinc hook region (amino acids 628-787) expressed in E. coli.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAD50 double strand break repair protein
• Database Link:
  NP_005723
  Entrez Gene 10111 Human
  UniProt ID Q92878
• Background: Rad50, a 153kDa protein, is associated with Mre11 and Nbs1 (Nibrin or p95) to form a multiprotein complex involved in the double strand break repair process. Rad50 is a member of the family of structural maintenance of chromosome (SMC) proteins. Members of this family share similar structures consisting of globular N- and C-termini separated by a coiled-coil domain. At the base of the coiled-coil region is a zinc binding motif, the zinc hook. The zinc hook mediates Rad50 dimerization through the linkage of two Rad50 coiled-coil tails.
• Synonyms: hRad50; NBSLD; RAD502

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination, Non-homologous end-joining