MEK4 (MAP2K4) Rabbit Polyclonal Antibody

CAT#: TA300116

Rabbit Polyclonal Antibody against MAP2K4

Product Images

Western blot analysis of MAP2K4 (arrow) using rabbit polyclonal MAP2K4 Antibody (S257) (Cat.#TA300116).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) (Cat.#TA300116), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50
• Reactivities: Human (Predicted: Mouse, Xenopus)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This MEKK4(MAP2K4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from human MEKK4(MAP2K4).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mitogen-activated protein kinase kinase 4
• Database Link:
  NP_003001
  Entrez Gene 26398 MouseEntrez Gene 6416 Human
  UniProt ID P45985
• Background: MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
• Synonyms: JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway