TRIAD3 (RNF216) (N-term) Rabbit Polyclonal Antibody

CAT#: SP2172P

TRIAD3 (RNF216) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of mouse heart lysate probed with Rabbit anti Mouse TRIAD3A at 0.5 (A), 1 (B) and 2 (C) ug/ml

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  Formalin fixed, paraffin embedded mouse heart stained with Rabbit anti Mouse TRIAD3A following antigen retrieval using sodium citrate buffer (pH6.0)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot (0.5 - 1.0 µg/ml, detects a band of approximately 95 kDa in mouse heart cell lysates).
  Immunohistochemistry on paraffin sections.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A 15 amino acid peptide located near mouse TRIAD3A amino-terminus
• Specificity: This antibody recognises an epitope within the N-terminal region (NT) of TRIAD3A.
• Formulation: Phosphate buffered saline, 0.02 % Sodium Azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Ion exchange chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Gene Name: ring finger protein 216
• Database Link:
  UniProt ID Q9NWF9
• Background: TRIAD3A is a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response.
  Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.
• Synonyms: RING finger protein 216, TRIAD3, UBCE7IP1, ZIN