Hamartin (TSC1) Rabbit Polyclonal Antibody

CAT#: SP2163P

Hamartin (TSC1) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunocytochemical staining of EL4 cells with Rabbit anti Human TSC1

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot (1 µg/ml, detects a band of approximately 130 kDa in mouse EL4 cell lysates).
  Immunocytochemistry.
  Has been reported as suitable for use in IHC on mouse EL4 cell cytospins at 2 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A 15 amino acid peptide located near the centre of human TSC1
• Specificity: This antibody recognises an epitope within the internal region of the 130 kDa tumour suppressor protein, Tumour sclerosis complex 1 (TSC1).
• Formulation: Phosphate buffered saline, 0.02 % Sodium Azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Gene Name: tuberous sclerosis 1
• Database Link:
  Entrez Gene 7248 Human
  UniProt ID Q92574
• Background: Mutation within the TSC1 gene is responsible for Tuberous sclerosis (TSC), an autosomal dominant genetic disorder associated with the development of benign tumors (hamartomas), a condition also induced by mutation of the Tumour sclerosis complex 2 (TSC2) gene.
  Many proteins have been shown to play a role in the regulation of the serine/threonine kinase known as target of rapamycin (TOR), a central component in a complex signaling pathway which controls cell proliferation and cell cycle progression. Negative regulation of TOR activity occurs following the over-expression of the TSC1 and TSC2 gene products, hamartin and tuberin. These act by suppressing the phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K), downstream targets of TOR involved in mRNA translation. Furthermore the TOR activator protein Rheb has been identified as a target of TSC1-TSC2 complexes.
• Synonyms: KIAA0243, TSC, Tuberous sclerosis 1 protein