DNA PKcs (PRKDC) Rabbit Polyclonal Antibody

CAT#: SP1136

DNA PKcs (PRKDC) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: EMSA, IP, WB
• Recommended Dilution: Western Blot: 1/2000.
  Immunoprecipitation.
  Gel Super Shift Assays.
  Immunohistochemistry on paraffin sections: 1:100; this product requires heat mediated treatment of paraffin sections; we recommend the use of antigen unmasking fluid for this purpose; a two hour incubation with SP1136 is recommended.
• Reactivities: Hamster, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human DNA-PKcs fragment
• Specificity: This antibody has a strong reactivity with DNA-PKcs.
• Formulation: State: Serum
  State: Liquid serum containing 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein kinase, DNA-activated, catalytic polypeptide
• Database Link:
  Entrez Gene 5591 Human
  UniProt ID P78527
• Background: DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.
• Synonyms: DNPK1, p460, HYRC, HYRC1