alpha Synuclein (SNCA) (61-95) Mouse Monoclonal Antibody [Clone ID: 5C2]

CAT#: SM6028

alpha Synuclein (SNCA) (61-95) mouse monoclonal antibody, clone 5C2, Purified

Product Images

Western blot analysis: The recombinant human synuclein family (a-, β-and ?-) and a-synuclein domains (1-60, 1-95, 61-140 and 96-140) proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-a-Synuclein (61-95 aa) antibody. (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• 

  Staining of Formalin-Fixed, Paraffin-Embedded Human Brain using SNCA antibody. at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Specifications

Product Data

• Clone Name: 5C2
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/2,000).
  Immunohistochemistry on Paraffin Sections (10 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human Alpha-Synuclein (61-95) purified from E. coli.
• Specificity: This antibody has the specificity against the NAC domain of Alpha-Synuclein (61-95).
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: synuclein alpha
• Database Link:
  Entrez Gene 6622 Human
  UniProt ID P37840
• Background: Alpha-Synuclein (amino acids 1-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. Alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-A beta component of amyloid plaques (NAC, amino acids 61-95) is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.
• Synonyms: NACP, PARK1