Tubulin (TUBA1B) Rat Monoclonal Antibody [Clone ID: YOL1/34]

CAT#: SM568P

Tubulin (TUBA1B) rat monoclonal antibody, clone YOL1/34, Purified

Product Images

Immunohistochemistry: Alpha Tubulin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: Alpha Tubulin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Nerve followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: Alpha Tubulin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Alpha Tubulin antibody staining of HeLa cells, green, nuclei are counterstained with DAPI.

Specifications

Product Data

• Clone Name: YOL1/34
• Applications: ELISA, IF, IHC, IP, R, WB
• Recommended Dilution: ELISA: 10 µg/ml as Detecting antibody.
  Radioimmunoassay.
  Western blotting.
  Immunofluorescence.
  Flow Cytometry (Not tested).
  Immunohistochemistry on Frozen and Paraffin Sections.
• Reactivities: Human, Mammalian, Mouse, Rat, Yeast, Birds, Drosophila
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Yeast Tubulin.
  Spleen cells from immunised LOU rats were fused with cells of the rat YB2/0 Myeloma cell line.
• Specificity: SM568P specifically recognizes the alpha subunit of Tubulin. The reactivity pattern is similar to that seen with Clone YL1/2 (cat.-No SM2202P).
  This antibody is routinely tested in ELISA on Tubulin.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction.
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tubulin alpha 1b
• Database Link:
  Entrez Gene 22143 MouseEntrez Gene 500929 RatEntrez Gene 10376 Human
  UniProt ID P68363
• Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
• Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection