CD4 Mouse Monoclonal Antibody [Clone ID: CVS4]

CAT#: SM497R

CD4 mouse monoclonal antibody, clone CVS4, PE, Purified

Product Images

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 antibody clone CVS4 Cat.-No SM497R.

Specifications

Product Data

• Clone Name: CVS4
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
• Reactivities: Equine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Equine thymocytes.
  Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
• Specificity: This anitbody CVS4 recognizes Equine CD4, a 58kDa cell surface glycoprotein that is primarily expressed on a subpopulation of equine T lymphocytes and considered to be the equine homologue of human CD4.
  A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).
• Formulation: Label: PE
  State: Purified
  State: Lyophilised purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID F6Y6X8
• Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
• Synonyms: T-cell surface antigen T4/Leu-3