CD4 Mouse Monoclonal Antibody [Clone ID: CC30]

CAT#: SM434PS

CD4 mouse monoclonal antibody, clone CC30, Purified

Specifications

Product Data

• Clone Name: CC30
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 10⁶ cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections.
  Clone CC30 has been reported as being suitable for use on Formalin Dichromate (FD5) Fixed Paraffin Embedded tissue with amplification and antigen retrieval techniques (See Ref.3 for details).
• Reactivities: Bovine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bovine thymocytes. Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody precipitates a molecule of approximately 50kDa and is considered to be the Bovine homologue of Human CD4.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 407098 Bovine
  UniProt ID A7YY52
• Background: The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.
• Synonyms: T-cell surface antigen T4/Leu-3