GST-Tag Mouse Monoclonal Antibody [Clone ID: S-tag-05]

CAT#: SM3155P

GST-Tag mouse monoclonal antibody, clone S-tag-05, Purified

Product Images

Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Western blot was immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02)

Specifications

Product Data

• Clone Name: S-tag-05
• Applications: IP, WB
• Recommended Dilution: Western blot: 1 µg/ml (Reducing Conditions).
  Positive Control: GST fusion protein.
  Immunoprecipitation.
• Reactivities: Escherichia coli, Mammalian, Schistosoma japonicum
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: GST fusion protein.
• Specificity: The GST-Tag monoclonal antibody S-tag-05 detects Glutathione-S-Transferase (GST) from Schistosoma japonicum. It is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, Yeast, Mammalian, and in vitro transcription/translation systems.
  Recognizes fusion proteins in all Species.
• Formulation: PBS, pH 7.4 with 0,05% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (>95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from desoatch.
• Database Link:
  UniProt ID P08515
• Background: The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
  Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
• Synonyms: Glutathione S-transferase Tag, GST26-Tag