IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: MEM-140]

CAT#: SM3151P

IL2 Receptor alpha (IL2RA) mouse monoclonal antibody, clone MEM-140, Purified

Product Images

Surface staining of CD25 in PHA activated PBMC with anti-CD25 (MEM-140) purified, GAM-APC.

Specifications

Product Data

• Clone Name: MEM-140
• Applications: FC, IP
• Recommended Dilution: Immunoprecipitation: excellent for immunoisolation of CD25+cells
  Flow Cytometry: 1 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: PHA-activated peripheral blood leucocytes
• Specificity: The antibody MEM-140 reacts with CD25 (Interleukin-2 receptor a chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
• Formulation: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Thiophilic adsorption-affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 2 receptor subunit alpha
• Database Link:
  Entrez Gene 3559 Human
  UniProt ID P01589
• Background: CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
• Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen