Glycophorin A (GYPA) (N-term) Mouse Monoclonal Antibody [Clone ID: HIR2]

CAT#: SM3141R

CD235ab (N-term) mouse monoclonal antibody, clone HIR2, PE

Product Images

Surface staining of human peripheral blood with anti-CD235a (HIR2) PE.

Specifications

Product Data

• Clone Name: HIR2
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (Human, N-terminal)
• Specificity: The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: PE
  State: Liquid Ig fraction
  Label: Conjugated with R-Phycoerythrin
• Purification: Size-exclusion chromatography
• Conjugation: PE
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycophorin A (MNS blood group)
• Database Link:
  Entrez Gene 2993 Human
  UniProt ID P02724
• Background: CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
• Synonyms: Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein