SLP76 (LCP2) Mouse Monoclonal Antibody [Clone ID: SLP-76/03]

CAT#: SM3135P

SLP76 (LCP2) mouse monoclonal antibody, clone SLP-76/03, Purified

Product Images

Western Blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03). Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line;Lane 2: JURKAT human peripheral blood T cell leukemia cell line;Lane 3: RAMOS human Burkitt lymphoma cell line

Specifications

Product Data

• Clone Name: SLP-76/03
• Applications: IHC, WB
• Recommended Dilution: Western Blot.
  Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, Positive tissue: thymus.
• Reactivities: Human, Porcine, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: A fusion protein representing amino acids 216-434 of human SLP-76
• Specificity: The antibody reacts with SLP76, a 76 kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: lymphocyte cytosolic protein 2
• Database Link:
  Entrez Gene 3937 Human
  UniProt ID Q13094
• Background: SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-g. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
• Synonyms: LCP2, Lymphocyte cytosolic protein 2, SLP-76 tyrosine phosphoprotein, SLP76