STAT1 pSer727 Mouse Monoclonal Antibody [Clone ID: PSM1]

CAT#: SM3126P

STAT1 pSer727 mouse monoclonal antibody, clone PSM1, Purified

Product Images

Western Blotting analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-gamma treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate

Specifications

Product Data

• Clone Name: PSM1
• Applications: IP, WB
• Recommended Dilution: Immunoprecipitation.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: STAT 1 peptide sequence 721-733 (Ser727 phosphorylated).
• Specificity: The antibody PSM1 recognizes STAT1 (91 kDa) activated by phosphorylation at Ser727.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified IgG fraction (> 95% by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: signal transducer and activator of transcription 1
• Database Link:
  Entrez Gene 6772 Human
  UniProt ID P42224
• Background: STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
• Synonyms: DKFZp686B04100; ISGF-3; OTTHUMP00000205845; STAT91