CD8A Mouse Monoclonal Antibody [Clone ID: MEM-31]

CAT#: SM3102APC

CD8A mouse monoclonal antibody, clone MEM-31, APC

Product Images

Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE

Specifications

Product Data

• Clone Name: MEM-31
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
  The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Crude thymus membrane fraction
• Specificity: This antibody recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8α homodimer or CD8α/β heterodimer (each monomer approx. 32-34 kDa).
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: APC
  State: Liquid Ig fraction
  Label: Conjugated with cross-linked Allophycocyanin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: APC
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8a molecule
• Database Link:
  Entrez Gene 925 Human
  UniProt ID P01732
• Background: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as αβ heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as αα homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
• Synonyms: CD8 alpha chain, CD8A, MAL

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway