Blood Group Lewis a Antigen Mouse Monoclonal Antibody [Clone ID: 7LE]

CAT#: SM3091P

Blood Group Lewis a Antigen mouse monoclonal antibody, clone 7LE, Purified

Product Images

Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).

Specifications

Product Data

• Clone Name: 7LE
• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry (paraffin section): 5 μg/ml (antigen retrieval with sodium citrate).
  Positive control:colorectal adenocarcinoma.
  Western Blotting.
  ELISA.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)
• Specificity: The antibody recognizes Lewis a blood group antigen.
  Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity: > 95 % (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Lewis a blood group antigen is a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells.