ICAM3 Mouse Monoclonal Antibody [Clone ID: MEM-171]

CAT#: SM3028P

ICAM3 mouse monoclonal antibody, clone MEM-171, Purified

Product Images

Surface staining of JURKAT human leukemia T cell line with anti-human CD50 (MEM-171) FITC.

Specifications

Product Data

• Clone Name: MEM-171
• Applications: FC, IP
• Recommended Dilution: Flow cytometry: 4 µg/ml.
  Immunoprecipitation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human granulocytes
• Specificity: The antibody recognizes an epitope in the D2 domain of human CD50 (ICAM-3).
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: intercellular adhesion molecule 3
• Database Link:
  Entrez Gene 3385 Human
  UniProt ID P32942
• Background: CD50 is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
• Synonyms: ICAM-3, ICAM-R, CDw50