CD34 Class III Mouse Monoclonal Antibody [Clone ID: 4H11]

CAT#: SM3018BT

CD34 Class III mouse monoclonal antibody, clone 4H11, Biotin

Product Images

Fig. 1. Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: 4H11
• Applications: FC
• Recommended Dilution: Indirect immunofluorescence analysis by Flow cytometry (1:200 as starting point).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
• Specificity: This antibody reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  Label: Biotin
  State: Liquid purified Ig fraction
  Label: The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: CD34 molecule
• Database Link:
  Entrez Gene 947 Human
  UniProt ID P28906
• Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
• Synonyms: Hematopoietic progenitor cell marker

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Hematopoietic cell lineage