CD3E Mouse Monoclonal Antibody [Clone ID: MEM-57]

CAT#: SM3017P

CD3E mouse monoclonal antibody, clone MEM-57, Purified

Product Images

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.

Specifications

Product Data

• Clone Name: MEM-57
• Applications: FC, FN, IP
• Recommended Dilution: Flow Cytometry: 2-5 µg/ml
  Positive Control: Peripheral Blood Lymphocytes, Jurkat T cell line.
  Immunoprecipitation: The antibody immuno-precipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7).
  Functional Application: MEM-57 activates T lymphocytes in vitro. It has a strong Mitogenic effect, it reacts strongly with Gamma/Delta Lymphocytes.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human thymocytes and T lymphocytes
• Specificity: The antibody reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
• Formulation: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD3e molecule
• Database Link:
  Entrez Gene 916 Human
  UniProt ID P07766
• Background: CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
  The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
• Synonyms: T3/Leu-4