Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: MEM-101A]

CAT#: SM3016R

Integrin beta 1 (ITGB1) mouse monoclonal antibody, clone MEM-101A, PE

Product Images

Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.

Specifications

Product Data

• Clone Name: MEM-101A
• Applications: FC
• Recommended Dilution: Flow Cytometry: 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human, Porcine, Canine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Raji Burkitt's lymphoma cell line
• Specificity: The antibody MEM-101A reacts with CD29 (integrin beta-1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: PE
  State: Liquid purified IgG fraction
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit beta 1
• Database Link:
  Entrez Gene 3688 Human
  UniProt ID P05556
• Background: CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 dibberent alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.
• Synonyms: Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12