CD14 Mouse Monoclonal Antibody [Clone ID: MEM-18]

CAT#: SM3009APC

CD14 mouse monoclonal antibody, clone MEM-18, APC

Product Images

Fig. 1. Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). Lane 1: whole cell lysate HEK 293 transfected with empty vector; Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA; Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA

• 

  Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) APC.

Specifications

Product Data

• Clone Name: MEM-18
• Applications: FC, WB
• Recommended Dilution: Suitable for Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
• Specificity: The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: APC
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: APC
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: One year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipo-polysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipo-polysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
• Synonyms: CD14