Sirpa Mouse Monoclonal Antibody [Clone ID: OX-41]

CAT#: SM275P

Sirpa mouse monoclonal antibody, clone OX-41, Purified

Specifications

Product Data

• Clone Name: OX-41
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot.
  Flow Cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunohistochemistry on frozen and paraffin sections: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with the NSO/U mouse myeloma cell line.
• Specificity: This antibody recognises CD172a, also known as Signal regulatory protein (SIRP). MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: signal-regulatory protein alpha
• Database Link:
  Entrez Gene 25528 Rat
  UniProt ID P97710
• Background: CD172a is expressed selectively by myeloid cells and by neurons.
• Synonyms: SHP substrate 1, SHPS-1, Sirp-alpha-2, Sirp-alpha-3, MyD-1 antigen, p84, BIT, MFR, MYD1, PTPNS1, SHPS1