Ptprc Mouse Monoclonal Antibody [Clone ID: OX-1]

CAT#: SM274R

Ptprc mouse monoclonal antibody, clone OX-1, PE

Product Images

Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD45 -RPE (Cat.-No SM274R).

Specifications

Product Data

• Clone Name: OX-1
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
  (This product is routinely tested in flow cytometry on rat splenocytes).
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat thymocyte membrane glycoproteins.
• Specificity: This antibody recognizes CD45.
  OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
• Formulation: PBS, pH 7.4
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water.
• Conjugation: PE
• Storage: Store undiluted Prior to and following reconstitution at 2-8°C.
  DO NOT FREEZE.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: One year from despatch.
• Gene Name: protein tyrosine phosphatase, receptor type, C
• Database Link:
  Entrez Gene 24699 Rat
  UniProt ID P04157
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200