Itgb1 Hamster Monoclonal Antibody [Clone ID: HM beta 1.1]

CAT#: SM2210F

Itgb1 hamster monoclonal antibody, clone HM beta 1.1, FITC

Product Images

Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29:FITC.

Specifications

Product Data

• Clone Name: HM beta 1.1
• Applications: FC
• Recommended Dilution: Flow Cytometry (Neat-1/10): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse, Rat
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Purified mouse VLA-4 antigen.
  Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
• Specificity: This antibody recognises the integrin beta 1 subunit (CD29).
  Clone HM beta 1.1 is reported to inhibit beta 1 integrin mediated adhesion. We recommend the use of SM2210LE for this purpose.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin beta 1 (fibronectin receptor beta)
• Database Link:
  Entrez Gene 16412 Mouse
  UniProt ID P09055
• Background: CD29 is a 110KD cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.
• Synonyms: Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12