HLA Class II DR Rat Monoclonal Antibody [Clone ID: YE2/36-HLK]

CAT#: SM2200P

HLA Class II DR rat monoclonal antibody, clone YE2/36-HLK, Purified

Specifications

Product Data

• Clone Name: YE2/36-HLK
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunohistochemistry on frozen and paraffin sections: This clone has been described reacting with paraffin embedded material following PLP (periodate-lysineparaformaldehyde) fixation.
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: EHR-B Ramos cells. Spleen cells from immunised AO rats were fused with cells of the rat Y3 Ag1.2.3 myeloma cell line.
• Specificity: This antibody recognises a monomorphic determinant of HLA-DR antigen (MHC class II). Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB.
• Formulation: State: Purified
  State: Liquid purified IgG containing 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: HLA DR is expressed by B lymphocytes, activated T cells and by monocytes.
• Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR