Chimaeric Human IgE anti Nitrophenyl-hapten (NP) Human Monoclonal Antibody [Clone ID: JW8/1]

CAT#: SM2177

Chimaeric Human IgE anti Nitrophenyl-hapten (NP) human monoclonal antibody, clone JW8/1, Supernatant

Specifications

Product Data

• Clone Name: JW8/1
• Applications: ELISA
• Recommended Dilution: Suitable for ELISA (1/20-1/100).
• Host: Human
• Isotype: IgE
• Clonality: Monoclonal
• Immunogen: Hapten, 4-hydroxy-3-nitrophenylacetyl (NP)
• Specificity: The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures.
  The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgE antibody specific for NP has been produced.
• Formulation: Contains 0.2M Tris/HCl pH7.4 and 5-10% Foetal Calf Serum in PBS buffer pH 7.2 with 0.09% Sodium Azide as preservative.
  State: Supernatant
  State: Liquid Tissue Culture Supernatant.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.