Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: BA-17]

CAT#: SM1822PS

Cytokeratin 19 (KRT19) mouse monoclonal antibody, clone BA-17, Purified

Product Images

Detection of cytokeratin 19 in MCF-7 cell lysate by mouse monoclonal antibody BA-17.

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  Immunohistochemistry: KRT19 antibody staining of Formalin-Fixed, paraffin-Eembedded Human Breast at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: KRT19 antibody staining of Formalin-Fixed, paraffin-Eembedded Human Pancreas at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: BA-17
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Western blotting.
  Flow Cytometry.
  Immunoprecipitation.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100. this product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
  Recommended Positive Control Tissue: Normal breast.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mammary organoids
• Specificity: The antibody reacts with Cytokeratin 19 (40 kDa) in Human Tissue.
  Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with Cytokeratin 7.
• Formulation: Phosphate buffered saline (PBS), pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 19
• Database Link:
  Entrez Gene 3880 Human
  UniProt ID P08727
• Background: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
• Synonyms: Cytokeratin-19, Keratin-19, KRT19, CK19, K19