LOC100507703 Mouse Monoclonal Antibody [Clone ID: BB7.2]

CAT#: SM1818P

LOC100507703 mouse monoclonal antibody, clone BB7.2, Purified

Specifications

Product Data

• Clone Name: BB7.2
• Applications: FC, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Recommended Positive Control: Tonsil.
  Flow Cytometry: Use 10 µl of 10 µg/ml diluted antibody to label 10e6 cells or 100 µl whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Papain solubilized HLA-A2.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises the HLA-A2 histocompatability antigen.
  The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. This antibody may be used for the Flow Cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P01892
• Background: Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).
• Synonyms: HLAA2, MHC class I antigen A-2