CD62L (SELL) Mouse Monoclonal Antibody [Clone ID: FMC46]

CAT#: SM1149P

CD62L (SELL) mouse monoclonal antibody, clone FMC46, Purified

Specifications

Product Data

• Clone Name: FMC46
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100; use 10 µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunoprecipitation.
  Immunohistochemistry on frozen sections.
  Recommended positive control tissue: Human Tonsil.
• Reactivities: Bovine, Human, Monkey
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: PHA stimulated lymphoblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognizes CD62L, also known a L-selectin.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: selectin L
• Database Link:
  Entrez Gene 6402 Human
  UniProt ID P14151
• Background: CD62L is a 74-95kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62-P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes. CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naive lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains. The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.
• Synonyms: SELL, LNHR, LYAM1, Leu-8, TQ1, gp90-MEL, LECAM1, LAM-1