Cd8a Rat Monoclonal Antibody [Clone ID: YTS105.18]

CAT#: SM024LE

Cd8a rat monoclonal antibody, clone YTS105.18, Low Endotoxin

Specifications

Product Data

• Clone Name: YTS105.18
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse spleen cells.
  Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
• Specificity: SM024LE recognises a non polymorphic epitope on the mouse CD8 alpha chain.
  Antibodies produced by clone YTS105.18 have been reported to block MHC I dependent T-cell responses in vitro and in vivo, and induce transplantation tolerance in combination with CD4 antibodies.
• Formulation: PBS, pH 7.4 without preservatives.
  State: Low Endotoxin
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted -20°C only.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8 antigen, alpha chain
• Database Link:
  Entrez Gene 12525 Mouse
  UniProt ID P01731
• Background: CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds. It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.
• Synonyms: CD8 alpha chain, CD8A, MAL