Biotin Goat Polyclonal Antibody

CAT#: R1615TR

Biotin goat polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Flow cytometry: 1.2,000 - 1:10,000.
  IF microscopy: 1:500 - 1:2,500.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotinylated bovine serum albumin and biotinylated IgG.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: Texas Red
  State: Lyophilized purified IgG fraction
  Label: - Sulfonyl Chloride (TR; Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 4.8 moles Texas RedTM per mole of Goat IgG F(ab')2.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with 1.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H