PGA5 Goat Polyclonal Antibody

CAT#: R1106PS

PGA5 goat polyclonal antibody, Purified

Product Images

Western blot of goat anti-Pepsin antibody. Lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Pepsin primary antibody at 1/1,000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 35 kDa/35 kDa for Pepsin. Other band (s): None.

• 

  Immunohistochemistry of goat anti-pepsin antibody 1:100

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/50,000-1/200,000.
  Western blot: 1/5,000-1/20,000.
  Immunohistochemistry on Paraffin and Frozen sections: 1/200-1/1,000.
  Immunoprecipitation: 1/100.
• Reactivities: Porcine
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Pepsin from porcine stomach
• Specificity: This antibody detects porcine Pepsin. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-goat serum as well as purified and partially purified Pepsin [porcine stomach].
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 396892 Pig
  UniProt ID P00791
• Background: Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.
• Synonyms: Pepsin A, PGA, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I, Pepsinogen A, PGA3, PGA4, PGA5