LPO Sheep Polyclonal Antibody

CAT#: R1095BS

LPO sheep polyclonal antibody, Biotin, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500 - 1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000 - 1/20,000.
  This product has been assayed against 1.0 µg of Lactoperoxidase [bovine milk] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/6,000 of the reconstitution concentration is suggested.
• Reactivities: Bovine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Lactoperoxidase from bovine milk
• Specificity: This product detects bovine Lactoperoxidase. Cross reactivity against Lactoperoxidase from other sources is unknown.
  Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-sheep serum as well as purified and partially purified Lactoperoxidase [bovine milk].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 280844 Bovine
  UniProt ID P80025
• Synonyms: LPO, SAPX, Lactoperoxidase, EC=1.11.1.7, Salivary peroxidase, SPO