HXK1 Rabbit Polyclonal Antibody

CAT#: R1093HRPS

HXK1 rabbit polyclonal antibody, HRP

Product Images

Western blot of rabbit anti-Hexokinase antibody peroxidase conjugated. Lane 1: Hexokinase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Hexokinase antibody peroxidase conjugated at 1/1,000 overnight at 4°C. Secondary antibody: n/a, blocking buffer for 30 min at RT. Predicted/Observed size: 54 kDa, 54 kDa for Hexokinase.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/1,000.
  ELISA: 1/1,000-1/2,500.
  This product has been assayed against 1.0 µg of Hexokinase [Yeast] in a standard capture ELISA using peroxidase conjugated strepavidin and ABTS as a substrate for 30 minutes at room temperature.
• Reactivities: Yeast
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Hexokinase from Yeast
• Specificity: This antibody detects Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Hexokinase [Yeast].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P04806
• Synonyms: HXK1, HKA, Hexokinase PI, Hexokinase-A