GST-Tag Goat Polyclonal Antibody

CAT#: R1088

GST-Tag goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western: 1/1,000-1/5,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoprecipitation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,500 to 1/8,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Schistosoma japonicum
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Glutathione-S-Transferase [Schistosoma japonicum].
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by Dlipidation and Defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P08515
• Background: GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
• Synonyms: Glutathione S-transferase Tag, GST26-Tag